Molecular Cloning has served as the foundation of technicalexpertise in labs worldwide for 30 years. No other manual has beenso popular, or so influential. Molecular Cloning, Fourth Edition,by the celebrated founding author Joe Sambrook and new co-author,the distinguished HHMI investigator Michael Green, preserves thehighly praised detail and clarity of previous editions and includesspecific chapters and protocols commissioned for the book fromexpert practitioners at Yale, U Mass, Rockefeller University, TexasTech, Cold Spring Harbor Laboratory, Washington University, andother leading institutions. The theoretical and historicalunderpinnings of techniques are prominent features of thepresentation throughout, information that does much to helptrouble-shoot experimental problems.For the fourth edition of this classic work, the content hasbeen entirely recast to include nucleic-acid based methods selectedas the most widely used and valuable in molecular and cellularbiology laboratories.Core chapters from the third edition have been revised tofeature current strategies and approaches to the preparation andcloning of nucleic acids, gene transfer, and expression analysis.They are augmented by 12 new chapters which show how DNA, RNA, andproteins should be prepared, evaluated, and manipulated, and howdata generation and analysis can be handled.The new content includes methods for studying interactionsbetween cellular components, such as microarrays, next-generationsequencing technologies, RNA interference, and epigenetic analysisusing DNA methylation techniques and chromatin immunoprecipitation.To make sense of the wealth of data produced by these techniques, abioinformatics chapter describes the use of analytical tools forcomparing sequences of genes and proteins and identifying commonexpression patterns among sets of genes.Building on thirty years of trust, reliability, and authority,the fourt