From the perspective of embryologists, the author uses vivid and interesting language to describe the controversial topics in the basic operation of assisted reproductive laboratory and the aspects that should be paid attention to, and puts forward new solutions. This book describes the laboratory layout, embryo culture, egg collection, laboratory instruments and tools, cryopreservation and other aspects of assisted reproductive laboratory operation. At the same time, it discusses the research direction of assisted reproductive technology and related issues of academic exchanges, and systematically explains the current situation, practical problems and responsibilities of embryologists in the field of experimental embryology. This book is a novel and unique operation guide for assisted reproductive technology laboratory, which can provide readers with multi-level interpretation of the comparison of Chinese and foreign experimental technology and related concepts, and can also be used as a reference guide for technicians, embryologists or related researchers of assisted reproductive embryo laboratory.